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antibodies against p atm  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antibodies against p atm
    Antibodies Against P Atm, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+atm/pmc13011042-69-21-25
    Average 86 stars, based on 1 article reviews
    antibodies against p atm - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    other:

    Article Title: Homoisoflavanone Delays Colorectal Cancer Progression via DNA Damage‐Induced Mitochondrial Apoptosis and Parthanatos‐Like Cell Death
    Article Snippet: Protein concentrations were determined by BCA assay (P0012, Beyotime).

    Article Title: Low dose radiation induces new protein synthesis that promotes cancer radiotherapy resistance.
    Article Snippet: Radiotherapy (RT) is a cornerstone of cancer treatment; however, its efficacy is frequently hampered by its adverse effects on normal tissues.. By studying the effects of high-dose radiotherapy (HDRT) and low-dose radiotherapy (LDRT), we found that cancer cells adapt distinct responses to these doses to reduce cytotoxicity.. Upon HDRT, cancThese authors contributed equally to this worker cells initiate a strong DNA damage response (DDR) to gain resistance through rapid production and/or activation of proteins for cell cycle arrest and DNA damage repair.

    Article Title: Novel imidazotetrazine derivatives overcome temozolomide resistance in glioblastoma by inducing ferroptosis and apoptosis
    Article Snippet: Antibodies against GAPDH (5174S), caspase 3 (9662S), cleaved caspase 3 (9661S), caspase 7 (9492S), cleaved caspase 7 (9491S), caspase 9 (9502S), cleaved caspase 9 (9505S), cleaved PARP (5625S), CDK1 (CDC2, 9116S), p-CDK1 (p-CDC2, 9114S), cyclin B1 (12231 T), P53 (2524S), ATM (2873 T), p-ATM (4526 T), and γ-H2AX (80312S), and anti-rabbit IgG, HRP-linked antibody (7074S), and anti-mouse IgG, and horseradish peroxidase (HRP)-linked antibody (7076S) were procured from Cell Signaling Technology (Beverly, MA, USA).

    Cell-Signaling:

    Article Title: SKA2 promotes gastric cancer progression by regulating glutathione metabolism
    Article Snippet: ATM , Cell Signaling Technology , Cat# 2873; RRID: AB_2062659. .. p-ATM , Cell Signaling Technology , Cat# 5883; RRID: AB_10835213. .. p-chk2 , Cell Signaling Technology , Cat# 2197; RRID: AB_2080501.

    Article Title: Inhibition of NAMPT targets DNA damage response to sensitize alkylating chemotherapy in TP53 mutant mantle cell lymphoma
    Article Snippet: Images were then imported into the 3DHISTECH CaseViewer 2.4 software (Hungary) for quantitative analysis, and a series of high-resolution .tif files were exported for further processing. .. Western blotting (WB) was performed to evaluate the expression levels of total protein and phospho-specific isoforms using the following antibodies: FANCD2 (Santa Cruz Biotechnology, sc-20022), RAD51 (Santa Cruz Biotechnology, sc-398587), PBEF (Santa Cruz Biotechnology, sc-166946), cleaved Caspase3 (Cell Signaling Technology, 9664s), PARP (Cell Signaling Technology, 9532s), cleaved PARP (Cell Signaling Technology, 5625s), γ-H2AX (Ser139; Cell Signaling Technology, 9718s), p-CHK1 (Ser345; Cell Signaling Technology, 2341s), p53 (Cell Signaling Technology, 2527s), p-ATR (Ser428; Cell Signaling Technology, 2853s), p-ATM (Ser1981; Cell Signaling Technology, 4526s), and p-CHK2 (Thr68; Cell Signaling Technology, 2197s). .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Cell Signaling Technology, 2118) and α-tubulin (Santa Cruz Biotechnology, sc8035) were used as loading controls.

    Western Blot:

    Article Title: Inhibition of NAMPT targets DNA damage response to sensitize alkylating chemotherapy in TP53 mutant mantle cell lymphoma
    Article Snippet: Images were then imported into the 3DHISTECH CaseViewer 2.4 software (Hungary) for quantitative analysis, and a series of high-resolution .tif files were exported for further processing. .. Western blotting (WB) was performed to evaluate the expression levels of total protein and phospho-specific isoforms using the following antibodies: FANCD2 (Santa Cruz Biotechnology, sc-20022), RAD51 (Santa Cruz Biotechnology, sc-398587), PBEF (Santa Cruz Biotechnology, sc-166946), cleaved Caspase3 (Cell Signaling Technology, 9664s), PARP (Cell Signaling Technology, 9532s), cleaved PARP (Cell Signaling Technology, 5625s), γ-H2AX (Ser139; Cell Signaling Technology, 9718s), p-CHK1 (Ser345; Cell Signaling Technology, 2341s), p53 (Cell Signaling Technology, 2527s), p-ATR (Ser428; Cell Signaling Technology, 2853s), p-ATM (Ser1981; Cell Signaling Technology, 4526s), and p-CHK2 (Thr68; Cell Signaling Technology, 2197s). .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Cell Signaling Technology, 2118) and α-tubulin (Santa Cruz Biotechnology, sc8035) were used as loading controls.

    Expressing:

    Article Title: Inhibition of NAMPT targets DNA damage response to sensitize alkylating chemotherapy in TP53 mutant mantle cell lymphoma
    Article Snippet: Images were then imported into the 3DHISTECH CaseViewer 2.4 software (Hungary) for quantitative analysis, and a series of high-resolution .tif files were exported for further processing. .. Western blotting (WB) was performed to evaluate the expression levels of total protein and phospho-specific isoforms using the following antibodies: FANCD2 (Santa Cruz Biotechnology, sc-20022), RAD51 (Santa Cruz Biotechnology, sc-398587), PBEF (Santa Cruz Biotechnology, sc-166946), cleaved Caspase3 (Cell Signaling Technology, 9664s), PARP (Cell Signaling Technology, 9532s), cleaved PARP (Cell Signaling Technology, 5625s), γ-H2AX (Ser139; Cell Signaling Technology, 9718s), p-CHK1 (Ser345; Cell Signaling Technology, 2341s), p53 (Cell Signaling Technology, 2527s), p-ATR (Ser428; Cell Signaling Technology, 2853s), p-ATM (Ser1981; Cell Signaling Technology, 4526s), and p-CHK2 (Thr68; Cell Signaling Technology, 2197s). .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Cell Signaling Technology, 2118) and α-tubulin (Santa Cruz Biotechnology, sc8035) were used as loading controls.

    Incubation:

    Article Title: Homoisoflavanone Delays Colorectal Cancer Progression via DNA Damage-Induced Mitochondrial Apoptosis and Parthanatos-Like Cell Death.
    Article Snippet: Samples were analyzed using a CytoFLEX S flow cytometer (Beckman Coulter), and data were processed using FlowJo. .. The membranes were then blocked with a 5% non-fat milk TBST (TBS containing 0.1% Tween-20) for 1 h at room temperature and incubated at 4◦C for overnight with primary antibodies against the following proteins: cleaved Caspase 3 (25128-1-AP, Proteintech), cleaved Caspase 8 (9496, CST), PARP (9542, CST), cleaved PARP (9541, CST), P21 (2947, CST), P53 (2527, CST), Cyclin A2 (91500, CST), Cyclin D1 (2978, CST), CDK1 (bs-1341R, Bioss), CDK2 (bs-10726R, Bioss), CDK7 (bs-0569R, Bioss), AKT (4691, CST), p-AKT (4060, CST), ERK (9102, CST), p-ERK (9101, CST), MEK (9126, CST), p-MEK (9154, CST), ATR (13934, CST), p-ATR (2853, CST), Chk1 (2360, CST), p-Chk1 (2348, CST), RAD51 (14961-1-AP, Proteintech), ATM (27156-1-AP, Proteintech), p-ATM (5883, CST), γ-H2AX (9718, CST), AIF (5318, CST), MIF (75038, CST), Cyt c (10993-1-AP, Proteintech), Lamin B (66095-1-Ig, Proteintech), VDAC1 (81538-1-RR, Proteintech) and GAPDH (60004-1-Ig, Proteintech). .. The cells were washed with PBS and stained with propidium iodide (F10797, Invitrogen) for 1 h at 4◦C in the dark.



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    Knocking down SKA2-induced cell-cycle arrest and apoptosis through <t>the</t> <t>SKA2/ROS/ATM</t> axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.
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    Image Search Results


    Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

    Journal: iScience

    Article Title: SKA2 promotes gastric cancer progression by regulating glutathione metabolism

    doi: 10.1016/j.isci.2026.115202

    Figure Lengend Snippet: Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

    Article Snippet: p-ATM , Cell Signaling Technology , Cat# 5883; RRID: AB_10835213.

    Techniques: Western Blot, Expressing, Knockdown, Over Expression, Control